Cyclin E2 抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
Cyclin E2 抗体

Cyclin E2 抗体

商家询价

产品名称： Cyclin E2 抗体

英文名称： Cyclin E2 Antibody

产品编号： XY11935-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 CYCLIN E2 抗体

Applications
In-house applications ELISA, WB, IF, FC, IHC     Application not tested? Please see our Guarantee.
Published applications WB Publications 4 citations –view here
Species specificity Human; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 11935-1-AP;Jurkat cellJurkat cells were subjected to SDS PAGE followed by western blot with 11935-1-AP(CCNE2 antibody) at dilution of 1:800
  • 11935-1-AP;HeLaImmunofluorescent analysis of HeLa cells using 11935-1-AP( CCNE2 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
  • 11935-1-AP;human testis tissueImmunohistochemistry of paraffin-embedded human testis tissue slide using 11935-1-AP( CCNE2 Antibody) at dilution of 1:50 (under 10x lens).
  • 11935-1-AP;human testis tissueImmunohistochemistry of paraffin-embedded human testis tissue slide using 11935-1-AP( CCNE2 Antibody) at dilution of 1:50 (under 40x lens).
  • 11935-1-AP;HeLa cells1X10^6 HeLa cells were stained with 0.2ug CCNE2 antibody (11935-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Positive WB detected in Jurkat cells
Positive IHC detected in Human testis tissue, human breast cancer tissue
Positive IF detected in HeLa cells
Positive FC detected in HeLa cells
Recommended dilution WB : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:20-1:200
 CYCLIN E2 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen Cyclin E2 fusion protein ag2532 Full name Cyclin E2
Calculated molecular
weight		 374aa,44 kDa Observed molecular 
weight		 44 kDa
GenBank accession 
number		 BC020729 Gene ID (NCBI) 9134
Gene symbol CCNE2 Synonyms CCNE2, CYCE2, cyclin E2, G1/S specific cyclin E2
Background CYCLIN E2 抗体

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells. CYCLIN E2 抗体

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